From these psychophysically determined equal wavelengths, the wavelength requiring the least amount of radiant power to be equal to the others was at 555 nm. Both of these equality methods were applied for pairs of wavelengths across the spectrum. The relative amount of radiant power needed by the test wavelength to be perceived as equal to the radiant power of the reference wavelength was then measured. ![]() The radiant power of the test was varied until the two wavelengths were judged equal (i.e., equal apparent brightness or no apparent flicker). For both techniques the radiant power of a reference wavelength was fixed and visually compared to a test wavelength with variable radiant power. One was based upon direct comparisons of the perceived brightness of two wavelengths while the other was based upon the disappearance of flicker for two rapidly oscillating wavelengths. Specifically, if any two monochromatic wavelengths were reported to be equal, it was assumed that both would have been stimulated by the same physical amount of light. Since sensitivity cannot be measured directly, the basic rationale for these experiments was to measure psychological judgements of equality for two monochromatic wavelengths and then piece together a relative sensitivity function from all wavelengths that had been judged as equal. ![]() The goal of one set of psychophysical experiments conducted in the early part of the twentieth century was to define light as a physical quantity for the emerging lighting industry by establishing a functional relationship between the radiant power of monochromatic wavelengths to the relative sensitivity of the human visual system to those wavelengths. Each of those behavioral responses will have a different functional relationship to the monochromatic wavelength exposure. For example, an exposure to monochromatic wavelength can evoke a reaction time, a subjective report of apparent brightness, a reported hue sensation, or suppression of melatonin synthesis. A wide variety of behavioral responses can be measured for the very same optical radiation incident on the retina in psychophysical experiments. Radiometry is the physical measurement of optical radiation. Toward that end, classic psychophysical methods can be used 7. To quantify light as a stimulus for the circadian system, it is necessary to develop a functional relationship between optical radiation incident on the retina and the spectral, temporal, and absolute responses of the SCN. ![]() This is true even though all retinal photoreceptors, including the intrinsically photosensitive retina ganglion cells (ipRGCs), participate in the various phototransduction processes for visual and non-visual systems 1, 2, 3, 4, 5, 6. It is now known that the spectral, temporal, spatial, and absolute sensitivity characteristics of the RHT neural channel stimulating the SCN are quite different from those exhibited by the optic nerve leading to visual functioning by the thalamus and visual cortex. The retinohypothalamic tract (RHT) is the direct neural pathway from the retina to the master biological clock, the suprachiasmatic nuclei (SCN) in the hypothalamus. The circadian system is perhaps one of the most important non-visual systems affected by retinal light exposure.
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